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Recombinant Mouse Ddc Protein, Myc/DDK-tagged

Cat.No. : Ddc-2490M
Product Overview : Purified recombinant protein of mouse full-length dopa decarboxylase (Ddc), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
  • Specification
  • Gene Information
  • Related Products
Description : Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 54.3 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Ddc dopa decarboxylase [ Mus musculus (house mouse) ]
Official Symbol : Ddc
Synonyms : DDC; dopa decarboxylase; aromatic-L-amino-acid decarboxylase; Aadc
Gene ID : 13195
mRNA Refseq : NM_016672
Protein Refseq : NP_057881
UniProt ID : O88533

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Why set the parameters of the DDC experiment? 07/17/2022

The parameters of the DDC experiment include capillary type, electric field strength, injection time, and extraction voltage. These parameters need to be optimized for the purpose of the experiment and the nature of the sample to ensure optimal ion extraction and mass spectrometry results.

How can I evaluate the reliability of the results of a DDC experiment? 07/16/2022

Assessing the reliability of DDC experimental results can be achieved by comparing the reproducibility and consistency of protein abundance in different samples. In addition, the accuracy of experimental results can also be assessed using multiple internal standard proteins.

How do I verify the results of a DDC experiment? 09/07/2021

Validation of DDC experimental results can be achieved by comparing the consistency and repeatability of the types and quantities of proteins identified in different samples. In addition, DDC results can also be validated using other protein analysis techniques, such as Western blot.

How can I improve the resolution of my DDC experiments? 03/06/2021

Improving the resolution of DDC experiments can be achieved by optimizing parameters such as electric field strength, injection time, and lead-out voltage. In addition, choosing the right capillary material and diameter can also affect the resolution.

Is it possible to quantify the results of DDC experiments? 01/13/2021

DDC results can be quantified by comparing the number of ions or protein abundance in different samples. Commonly used quantitative methods include internal standard method, external standard method, and isotope labeling method.

Can DDC experimental data be processed? 10/16/2020

DDC experimental data needs to be processed and analyzed by professional software. Commonly used data processing methods include background subtraction, noise filtering, ion peak identification, and mass spectral interpretation.

Customer Reviews (3)

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Reviews
12/23/2022

    At the same dose, the protein has high expression characteristics.

    11/16/2022

      The stability of this protein determines its easy storage properties.

      01/05/2022

        DDC has high sensitivity and low detection limit.

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