Recombinant Mouse Ep300 Protein, GST-tagged

• Cat.No.: Ep300-49M
• Product Overview: Recombinant mouse Ep300 protein with a C-terminal GST-tag was expressed in E. coli.

Specification

• Description: p300/(CREB binding protein) associated factor (PCAF) belongs to the GCN5/PCAF family of nuclear histone acetyltransferases (HATs). Gcn5/PCAF proteins contain a-160 residue HAT domain and a conserved bromodomain directly C-terminal to the HAT domain, which has been shown recently to be an acetyl-lysine targeting motif. PCAF acetylates specific lysines on the N-terminal tails of Histones H3 & H4 and on the transcriptional activators MyoD, E2F1, and p53. Recently, PCAF was also shown to acetylate PTEN on lysine residues K125 & K128 within the catalytic cleft thus inactivating the enzyme. In many, but not all of these cases, acetylation has been shown to enhance the DNA binding affinity of the affected protein. Although the recombinant HAT proteins will acetylate free histones, nucleosomal acetylation only occurs in the context of the in vivo HAT complex.
• Source: E. coli
• Species: Mouse
• Tag: GST
• Molecular Mass: 40 kDa
• Protein length: 165
• Purity: ≥80% estimated by SDS-PAGE
• Applications: Enzyme Activity
• Stability: ≥ 6 months
• Storage: Store it at -80 centigrade
• Storage Buffer: 50 mM sodium phosphate, pH 7.2, with 100 mM sodium chloride, 1 mM EDTA, and 20% glycerol

Gene Information

• Gene Name: Ep300 E1A binding protein p300 [ Mus musculus (house mouse) ]
• Official Symbol: Ep300
• Synonyms: Ep300; E1A binding protein p300; p300; KAT3B; p300 HAT; A430090G16; A730011L11; histone acetyltransferase p300; E1A-associated protein p300; histone butyryltransferase p300; histone crotonyltransferase p300; protein 2-hydroxyisobutyryltransferase p300; protein propionyltransferase p300
• Gene ID: 328572
• mRNA Refseq: NM_177821
• Protein Refseq: NP_808489
• UniProt ID: B2RWS6