Recombinant Mouse Exd1 Protein, Myc/DDK-tagged

• Cat.No.: Exd1-2884M
• Product Overview: Purified recombinant protein of mouse full-length exonuclease 3'-5' domain containing 1 (Exd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity. The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 64 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Exd1 exonuclease 3'-5' domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Exd1
• Synonyms: EXD1; exonuclease 3-5 domain containing 1; exonuclease 3-5 domain-containing protein 1; exonuclease 3-5 domain-like 1; Vexonuclease 3-5 domain-like 1; exonuclease 3-5 domain-like-containing protein 1; Exdl1; 4932702D22Rik
• Gene ID: 241624
• mRNA Refseq: NM_172857
• Protein Refseq: NP_766445
• UniProt ID: Q8CDF7