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Recombinant Mouse F7 protein, His-tagged

Cat.No. : F7-1912M
Product Overview : Recombinant Mouse F7 aa. (Ala42~Lys169 (Accession # P70375)) fused with N-terminal His tag was produced in E. coli cells.
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Description : This gene encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III (TF III), the encoded protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the encoded protein in mice results in in perinatal lethality due to bleeding from normal blood vessels.
Source : E. coli
Species : Mouse
Tag : His
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 16.2kDa.
Protein length : Ala42~Lys169 (Accession # P70375)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage Buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in ddH2O.
Gene Name : F7 coagulation factor VII [ Mus musculus (house mouse) ]
Official Symbol : F7
Synonyms : Pfkl; phosphofructokinase, liver type; PFK-L; ATP-PFK; ATP-dependent 6-phosphofructokinase, liver type; 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; PFK-B; phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphofructokinase, liver, B-type; phosphohexokinase
Gene ID : 14068
mRNA Refseq : NM_010172.4
Protein Refseq : NP_034302.2
UniProt ID : P70375

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How does F7 contribute to hemostasis, and what is its role in preventing excessive bleeding? 04/02/2021

Factor VII's role in the coagulation cascade ensures rapid initiation of clot formation, preventing excessive bleeding and contributing to hemostasis.

Discuss the role of F7 in the extrinsic pathway of blood coagulation. 07/20/2020

Factor VII is a key player in the extrinsic pathway, where it forms a complex with tissue factor to activate factor X, initiating the cascade leading to thrombin generation.

What are the mechanisms of F7 activation, and how is it regulated at the molecular level? 01/15/2019

Factor VII is activated through a proteolytic cleavage, converting it into its active form (VIIa). This activation is regulated by tissue factor and other coagulation factors.

Explain the biochemical basis of F7 deficiency and its implications for bleeding disorders. 11/05/2018

F7 deficiency results in impaired blood clotting, leading to a bleeding disorder known as coagulation factor VII deficiency. This condition can manifest in varying degrees of severity.

Explain the role of F7 in the coagulation cascade and blood clot formation. 09/28/2018

Factor VII is activated to its enzymatically active form (factor VIIa) and initiates the coagulation cascade by activating factor X, ultimately leading to the formation of a stable blood clot.

Customer Reviews (3)

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Reviews
05/18/2022

    Quick turnaround—this product arrived swiftly, facilitating our research.

    10/03/2019

      Exceptional quality and precision—our lab relies on this protein consistently.

      05/29/2018

        Invaluable assistance from customer service—professional and responsive.

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