Active Recombinant Mouse Fap protein, His-tagged

• Cat.No.: FAP-584M
• Product Overview: Recombinant Mouse Fap fused with His tag was expressed in HEK293F.

Specification

• Description: This gene belongs to the serine protease family. The encoded protein is an inducible cell-surface bound glycoprotein specifically expressed in tumor-associated fibroblasts and pericytes of epithelial tumors and has protease and gelatinase activity. The protein plays a role in remodeling of the extracellular matrix (ECM) and may affect tumorigenesis and tissue repair. Alternately spliced transcript variants of this gene are described in the literature (PMID 9139873), but the full-length sequence of these variants is not available.
• Source: HEK293F
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile 25mM Tris, 250mM NaCl, pH 8.2
• Bio-activity: The specific activity(kcat)is >1000 pmol/min/μg. kcat/Km>0.5×10E4 (M^-1s^-1)Measured by its ability to convert the substrate ZGPAMC to ZGlyPro and AMC.
• Molecular Mass: ~90 kDa
• AA Sequence: HHHHHHHHHHENLYFQLRPSRVYKP EGNTKRALTLKDILNGTFSYKTYFP NWISEQEYLHQSEDDNIVFYNIETR ESYIILSNSTMKSVNATDYGLSPDR QFVYLESDYSKLWRYSYTATYYIYD LQNGEFVRGYELPRPIQYLCWSPVG SKLAYVYQNNIYLKQRPGDPPFQIT YTGRENRIFNGIPDWVYEEEMLATK YALWWSPDGKFLAYVEFNDSDIPII AYSYYGDGQYPRTINIPYPKAGAKN PVVRVFIVDTTYPHHVGPMEVPVPE MIASSDYYFSWLTWVSSERVCLQWL KRVQNVSVLSICDFREDWHAWECPK NQEHVEESRTGWAGGFFVSTPAFSQ DATSYYKIFSDKDGYKHIHYIKDTV ENAIQITSGKWEAIYIFRVTQDSLF YSSNEFEGYPGRRNIYRISIGNSPP SKKCVTCHLRKERCQYYTASFSYKA KYYALVCYGPGLPISTLHDGRTDQE IQVLEENKELENSLRNIQLPKVEIK KLKDGGLTFWYKMILPPQFDRSKKY PLLIQVYGGPCSQSVKSVFAVNWIT YLASKEGIVIALVDGRGTAFQGDKF LHAVYRKLGVYEVEDQLTAVRKFIE MGFIDEERIAIWGWSYGGYVSSLAL ASGTGLFKCGIAVAPVSSWEYYASI YSERFMGLPTKDDNLEHYKNSTVMA RAEYFRNVDYLLIHGTADDNVHFQN SAQIAKALVNAQVDFQAMWYSDQNH GISSGRSQNHLYTHMTHFLKQCFSL SD
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: >95%, by SDS-PAGE under reducing conditions
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: Fap fibroblast activation protein [ Mus musculus ]
• Official Symbol: Fap
• Synonyms: FAP; fibroblast activation protein; seprase; integral membrane serine protease; fibroblast activation protein alpha
• Gene ID: 14089
• mRNA Refseq: NM_007986
• Protein Refseq: NP_032012
• UniProt ID: P97321
• Chromosome Location: 2 C1.3; 2 35.85 cM
• Function: endopeptidase activity; hydrolase activity; peptidase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity

Reviews

08/11/2020

It enables accurate detection and quantification of FAP protein levels in samples, providing crucial insights into its expression, regulation, and potential functional changes under different experimental conditions.

05/04/2020

FAP protein is highly recommended for researchers due to its excellent performance in various assays, particularly in Western blotting (WB) experiments, and its applicability in protein electron microscopy structure analysis.

01/02/2019

FAP protein is highly recommended for researchers, as it demonstrates excellent performance in WB experiments and has proven useful in protein electron microscopy structure analysis.

Q&As

How does FAP contribute to immunosuppression in the tumor microenvironment?

FAP can suppress the immune response by modulating the activity of certain immune cells, creating an immunosuppressive tumor microenvironment.

How can FAP expression be used as a prognostic marker in cancer patients?

High levels of FAP expression in tumors have been associated with poor prognosis, making it a potential prognostic marker for certain cancers.

Are there ongoing clinical trials exploring FAP-targeted therapies?

Yes, several clinical trials are investigating the safety and efficacy of FAP-targeted therapies in various cancer types.

Can FAP be utilized as a therapeutic target beyond cancer treatment?

FAP-targeted therapies are being explored for conditions such as fibrotic diseases and rheumatoid arthritis, extending its potential clinical applications.

How is imaging technology utilized to detect FAP expression in tumors?

Molecular imaging techniques, such as positron emission tomography (PET), can be employed to visualize FAP expression in tumors noninvasively.