| Bio-activity : |
Fibulin 2 (FBLN2) is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined. Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin. It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors. To test the effect of FBLN2 on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant mouse FBLN2 diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1h at 37°C. Proliferation of A549 cells after incubation with FBLN2 for 72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant mouse FBLN2 for 72h. The result was shown. It was obvious that FBLN2 significantly inhibit cell viability of A549 cells. The ED50 is 1.25 μg/mL. |
