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Recombinant Mouse Fen1 Protein, Myc/DDK-tagged

Cat.No. : Fen1-2986M
Product Overview : Purified recombinant protein of mouse full-length flap structure specific endonuclease 1 (Fen1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
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Description : Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 42.6 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Fen1 flap structure specific endonuclease 1 [ Mus musculus (house mouse) ]
Official Symbol : Fen1
Synonyms : FEN1; flap structure specific endonuclease 1; flap endonuclease 1; FEN-1; flap structure-specific endonuclease 1; AW538437
Gene ID : 14156
mRNA Refseq : NM_007999
Protein Refseq : NP_032025
UniProt ID : Q91Z50

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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How are mutations in the FEN1 protein detected and analyzed? 11/08/2022

Mutations in the FEN1 protein can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

Can FEN1 protein be used in medical therapy? 03/15/2021

The FEN1 protein can be used as a biomarker in medical therapy to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting the FEN1 protein, such as inhibiting its activity or regulating its expression, are also being investigated.

What disease might be indicative of abnormal levels of FEN1 protein? 09/10/2020

Abnormal levels of the FEN1 protein may suggest a genetic disorder such as cancer or neurofibroma, but it may also be associated with other diseases.

How to study the regulatory mechanism of FEN1 protein? 08/18/2020

Studying the regulatory mechanism of FEN1 protein requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

What diseases can FEN1 protein deficiency or aberrant expression cause? 06/13/2020

Deficiency or aberrant expression of FEN1 protein may be associated with some cancers and genetic diseases, such as lung cancer, bowel cancer, neurofibromas, etc.

What are the types of mutations in the FEN1 protein? 06/09/2020

The types of mutations in the FEN1 protein include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

Customer Reviews (3)

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Reviews
01/01/2022

    FEN1 has strong stability.

    12/31/2021

      It shows excellent stability in production.

      08/23/2021

        FEN1 is easy to operate.

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