Recombinant Mouse Fgf1 Protein Fgf1-057M

Recombinant Mouse Fgf1 Protein

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Recombinant Mouse Fgf1 Protein

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Cat.No. : Fgf1-057M
Product Overview : Purified recombinant protein of Mouse fibroblast growth factor 1 (Fgf1) without tag was expressed in E.coli.
Description : Broad expression in lung adult (RPKM 20.2), heart adult (RPKM 15.2) and 16 other tissues.
Source : E. coli
Species : Mouse
Bio-activity : Assay #1: ED50 as determined by the dose-dependent stimulation of thymidine uptake by 3T3 cells in the presence of heparin is less than or equal to 0.5 ng/ml corresponding to a specific activity of > 2 x 10^6 units/mg. Assay #2: ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is less than or equal to 0.2 ng/ml in the presence of 10 ug/mL heparin, corresponding to a specific activity of > 5 x 10^6 units/mg.
Molecular Mass : 15.9 kDa
AA Sequence : MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D
Endotoxin : Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
Purity : >95%, as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for at least 6 months from date of receipt under proper storage and handling conditions.
Storage : Store at -80 centigrade.
Storage Buffer : Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
Reconstitution : Resuspend the protein in the desired concentration in proper buffer.
Figure :

Gene Name : Fgf1 fibroblast growth factor 1 [ Mus musculus (house mouse) ]
Official Symbol : Fgf1
Synonyms : Fgf1; fibroblast growth factor 1; Fam; Fgfa; Dffrx; Fgf-1; fibroblast growth factor 1; HBGF-1; aFGF; acidic fibroblast growth factor; fibroblast growth factor 1 (acidic); heparin-binding growth factor 1
Gene ID : 14164
mRNA Refseq : NM_010197
Protein Refseq : NP_034327
UniProt ID : P61148

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