Recombinant Mouse Ficd Protein, Myc/DDK-tagged

• Cat.No.: Ficd-3015M
• Product Overview: Purified recombinant protein of mouse full-length FIC domain containing (Ficd), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP. In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 52.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ficd FIC domain containing [ Mus musculus (house mouse) ]
• Official Symbol: Ficd
• Synonyms: FICD; FIC domain containing; adenosine monophosphate-protein transferase FICD; AMPylator FICD; FIC domain-containing protein; huntingtin interactor protein E; huntingtin interacting protein E; Hype; D5Ertd40e; MGC117722
• Gene ID: 231630
• mRNA Refseq: NM_001010825
• Protein Refseq: NP_001010825
• UniProt ID: Q8BIX9