Recombinant Mouse Fktn Protein, Myc/DDK-tagged

• Cat.No.: Fktn-3034M
• Product Overview: Purified recombinant protein of mouse full-length fukutin (Fktn), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the transfer of CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). This constitutes the first step in the formation of the ribitol 5-phosphate tandem repeat which links the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1. Required for normal location of POMGNT1 in Golgi membranes, and for normal POMGNT1 activity. May interact with and reinforce a large complex encompassing the outside and inside of muscle membranes. Could be involved in brain development (Probable).
• Molecular Mass: 53.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Fktn fukutin [ Mus musculus (house mouse) ]
• Official Symbol: Fktn
• Synonyms: FKTN; fukutin; Fukuyama type congenital muscular dystrophy homolog; Fukuyama type congenital muscular dystrophy (fukutin); Fcmd; D830030O17Rik
• Gene ID: 246179
• mRNA Refseq: NM_139309
• Protein Refseq: NP_647470
• UniProt ID: Q8R507