Recombinant Mouse Gale Protein, Myc/DDK-tagged

• Cat.No.: Gale-3136M
• Product Overview: Purified recombinant protein of mouse full-length galactose-4-epimerase, UDP (Gale), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes two distinct but analogous reactions: the reversible epimerization of UDP-glucose to UDP-galactose and the reversible epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The reaction with UDP-Gal plays a critical role in the Leloir pathway of galactose catabolism in which galactose is converted to the glycolytic intermediate glucose 6-phosphate. It contributes to the catabolism of dietary galactose and enables the endogenous biosynthesis of both UDP-Gal and UDP-GalNAc when exogenous sources are limited. Both UDP-sugar interconversions are important in the synthesis of glycoproteins and glycolipids.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 38.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gale galactose-4-epimerase, UDP [ Mus musculus (house mouse) ]
• Official Symbol: Gale
• Synonyms: Gale; galactose-4-epimerase, UDP; AI323962; 2310002A12Rik; UDP-glucose 4-epimerase; UDP-GalNAc 4-epimerase; UDP-GlcNAc 4-epimerase; UDP-N-acetylglucosamine 4-epimerase; UDP-galactosamine 4-epimerase; UDP-galactose 4-epimerase; galactowaldenase; EC 5.1.3.2; EC 5.1.3.7
• Gene ID: 74246
• mRNA Refseq: NM_178389