Active Recombinant Mouse Gdf15 protein
Cat.No. : | Gdf15-393M |
Product Overview : | Recombinant Mouse Gdf15(Ser189-Ala303) was expressed in E. coli. |
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Description : | Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), Placental Transforming Growth Factor beta, Prostate-derived Factor, and Placental Bone Morphogenetic Protein, is a divergent member of the TGF-beta superfamily. Cellular responses to TGF-beta proteins are mediated by hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. Mature mouse GDF-15 shares 66% and 97% amino acid sequence identity with the human and rat proteins, respectively. The C-terminal domain of GDF-15 contains seven characteristic conserved cysteine residues necessary for the formation of the cysteine knot and the single inter-chain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein. GDF-15 has been shown to have various functions, including inhibition of TNF-alpha production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation. GDF-15 also promotes neuronal survival, and hypothalamic expression of GDF-15 causes appetite suppression via modulation of neuropeptide Y and pro-opiomelanocortin levels. GDF-15 is cardioprotective via inhibition of platelet activation, limiting atherosclerosis, promoting recovery following myocardial infarction, and regulating angiogenesis. Exposure of cardiomyocytes to GDF-15 results in Smad2 and Smad3 phosphorylation. |
Source : | E. coli |
Species : | Mouse |
Predicted N Terminal : | Ser189 |
Form : | Lyophilized from a 0.2 μm filtered solution in HCl. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Mouse GSF-15 is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse Activin RIB/ALK-4 Fc Chimera that produces 50% of the optimal binding response is approximately 1-6 μg/mL. |
Molecular Mass : | Predicted Molecular Mass: 13 kDa; SDS-PAGE: 9-13 kDa, reducing conditions. |
Protein length : | Ser189-Ala303 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 250 μg/mL in 4 mM HCl. |
Shipping : | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Gene Name : | Gdf15 growth differentiation factor 15 [ Mus musculus ] |
Official Symbol : | Gdf15 |
Synonyms : | GDF15; growth differentiation factor 15; growth/differentiation factor 15; GDF-15; macrophage inhibiting cytokine-1; SBF; MIC-1; NAG-1; |
Gene ID : | 23886 |
mRNA Refseq : | NM_011819 |
Protein Refseq : | NP_035949 |
UniProt ID : | Q9Z0J7 |
Chromosome Location : | 8; 8 B3.3 |
Function : | growth factor activity; |
Products Types
◆ Recombinant Protein | ||
GDF15-0067H | Recombinant Human GDF15 Protein | +Inquiry |
GDF15-4821H | Active Recombinant Human GDF15 Protein | +Inquiry |
GDF15-204H | Recombinant Human GDF15 protein(Ala197-Ile308), hFc-tagged | +Inquiry |
GDF15-4822H | Recombinant Human GDF15 Protein, GST-tagged | +Inquiry |
GDF15-20H | Recombinant Human GDF15 protein(Ala197-Ile308), His-tagged | +Inquiry |
◆ Native Protein | ||
GDF15-27680TH | Active Native Human GDF15 Protein | +Inquiry |
◆ Lysates | ||
GDF15-2705HCL | Recombinant Human GDF15 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionGDF15 influences cardiovascular health, impacting heart failure progression and atherosclerotic processes.
GDF15 modulates inflammatory and immune responses, crucial in the context of chronic diseases.
GDF15 contributes to tissue repair and fibrosis, particularly in liver and kidney pathologies.
GDF15 affects cancer development by altering the tumor microenvironment and influencing tumor cell behavior.
Changes in GDF15 expression are linked to aging and age-related diseases, affecting various physiological processes.
GDF15 is involved in regulating energy metabolism and appetite, playing a key role in obesity and metabolic syndromes.
GDF15 is involved in cellular stress responses, influencing apoptosis and cell survival mechanisms.
Customer Reviews (3)
Write a reviewDependable protein purification, consistent and cost-effective.
Precise mass spectrometry analysis, key for proteomics research.
Great antibody validation, enhances our immunoprecipitation experiments.
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