Recombinant Mouse Gm2a Protein, Myc/DDK-tagged

• Cat.No.: Gm2a-3241M
• Product Overview: Purified recombinant protein of mouse full-length GM2 ganglioside activator protein (Gm2a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Binds gangliosides and stimulates ganglioside GM2 degradation. It stimulates only the breakdown of ganglioside GM2 and glycolipid GA2 by beta-hexosaminidase A. It extracts single GM2 molecules from membranes and presents them in soluble form to beta-hexosaminidase A for cleavage of N-acetyl-D-galactosamine and conversion to GM3. The large binding pocket can accommodate several single chain phospholipids and fatty acids, GM2A also exhibits some calcium-independent phospholipase activity. Has cholesterol transfer activity.
• Molecular Mass: 20.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gm2a GM2 ganglioside activator protein [ Mus musculus (house mouse) ]
• Official Symbol: Gm2a
• Synonyms: GM2A; GM2 ganglioside activator protein; ganglioside GM2 activator; shingolipid activator protein 3; cerebroside sulfate activator protein; SAP-3; GM2-AP; AA408702; AW215435
• Gene ID: 14667
• mRNA Refseq: NM_010299
• Protein Refseq: NP_034429
• UniProt ID: Q60648