Active Recombinant Mouse Gpt protein, His-tagged
Cat.No. : | Gpt-131M |
Product Overview : | Recombinant Mouse Gpt(1-496 aa) fused with His tag at N-terminal was expressed in E. coli. |
- Specification
- Gene Information
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Description : | Gpt, also known as alanine aminotransferases 1, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. |
Source : | E. coli |
Species : | Mouse |
Tag : | His |
Form : | Liquid. In Phosphate Buffered Saline (pH7.4) containing 1mM DTT, 10% glycerol |
Bio-activity : | Specific activity is > 40units/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C |
Molecular Mass : | 57.5 kDa (519 aa) confirmed by MALDI-TOF |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MGSMASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS |
Purity : | > 90% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Absorbance at 280nm ) |
Gene Name : | Gpt glutamic pyruvic transaminase, soluble [ Mus musculus ] |
Official Symbol : | Gpt |
Synonyms : | GPT; glutamic pyruvic transaminase, soluble; alanine aminotransferase 1; ALT1; GPT 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; ALT; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik; |
Gene ID : | 76282 |
mRNA Refseq : | NM_182805 |
Protein Refseq : | NP_877957 |
UniProt ID : | Q8QZR5 |
Chromosome Location : | 15 D3; 15 36.28 cM |
Pathway : | Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; |
Function : | L-alanine:2-oxoglutarate aminotransferase activity; catalytic activity; pyridoxal phosphate binding; transaminase activity; transferase activity; transferase activity, transferring nitrogenous groups; |
Products Types
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◆ Native Protein | ||
GPT-187H | Active Native Human Glutamate Pyruvate Transaminase | +Inquiry |
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GPT-26882TH | Native Human GPT | +Inquiry |
◆ Lysates | ||
GPT-721RCL | Recombinant Rat GPT cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionThe GPT gene, encompassing GPT1 and GPT2, is pivotal in glucose and amino acid metabolism. GPT2 is notably linked to tumorigenesis in various cancers, while the involvement of GPT1 in cancer remains uncertain and requires further exploration.
The GPT gene encodes ALT1, a key enzyme in cellular metabolism. ALT1 facilitates the reversible transamination between alanine and 2-oxoglutarate, crucial for glucose and amino acid metabolism. Elevated serum ALT1 levels are commonly used as a biomarker for liver injury caused by drug toxicity, infection, alcohol, and steatosis.
SMS protein alone doesn't impact LSCC patient prognosis, but when combined with GPT, it shows significance. Inconsistency between SMS transcriptional and protein levels may stem from various factors, including the involvement of regulatory elements and different post-transcriptional and post-translational modifications.
Analysis of LSCC tissues and cell lines revealed an inverse relationship between GPT and SMS expressions, confirmed through qRT-PCR and TCGA data. GPT protein, primarily located in the epithelium, was down-regulated in LSCC tissues.
GPT gene expression serves as a marker for cancer-induced metabolic reprogramming in tissues, indicating shifts in energy metabolism and biosynthetic processes.
Customer Reviews (3)
Write a reviewFantastic customer support. They genuinely care about the success of our research.
Top-notch protein quality! It's our go-to for reliable and reproducible results.
Very happy with this lab product!
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