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Active Recombinant Mouse Gpt protein, His-tagged

Cat.No. : Gpt-131M
Product Overview : Recombinant Mouse Gpt(1-496 aa) fused with His tag at N-terminal was expressed in E. coli.
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Description : Gpt, also known as alanine aminotransferases 1, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings.
Source : E. coli
Species : Mouse
Tag : His
Form : Liquid. In Phosphate Buffered Saline (pH7.4) containing 1mM DTT, 10% glycerol
Bio-activity : Specific activity is > 40units/mg, and is defined as the amount of
enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37°C
Molecular Mass : 57.5 kDa (519 aa) confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSMASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN
IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS
TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG
QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ
KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR
AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS
Purity : > 90% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Absorbance at 280nm )
Gene Name : Gpt glutamic pyruvic transaminase, soluble [ Mus musculus ]
Official Symbol : Gpt
Synonyms : GPT; glutamic pyruvic transaminase, soluble; alanine aminotransferase 1; ALT1; GPT 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; ALT; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik;
Gene ID : 76282
mRNA Refseq : NM_182805
Protein Refseq : NP_877957
UniProt ID : Q8QZR5
Chromosome Location : 15 D3; 15 36.28 cM
Pathway : Alanine and aspartate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, organism-specific biosystem; Alanine, aspartate and glutamate metabolism, conserved biosystem; Amino acid synthesis and interconversion (transamination), organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem;
Function : L-alanine:2-oxoglutarate aminotransferase activity; catalytic activity; pyridoxal phosphate binding; transaminase activity; transferase activity; transferase activity, transferring nitrogenous groups;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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What role does the GPT gene, including GPT1 and GPT2 subtypes, play in glucose and amino acid metabolism, and how is it implicated in cancer-related tumorigenesis? 05/01/2022

The GPT gene, encompassing GPT1 and GPT2, is pivotal in glucose and amino acid metabolism. GPT2 is notably linked to tumorigenesis in various cancers, while the involvement of GPT1 in cancer remains uncertain and requires further exploration.

What role does the GPT gene, encoding cytosolic alanine aminotransaminase 1 (ALT1), play in cellular metabolism and liver function? 04/13/2021

The GPT gene encodes ALT1, a key enzyme in cellular metabolism. ALT1 facilitates the reversible transamination between alanine and 2-oxoglutarate, crucial for glucose and amino acid metabolism. Elevated serum ALT1 levels are commonly used as a biomarker for liver injury caused by drug toxicity, infection, alcohol, and steatosis.

How does the combined SMS and GPT signature affect LSCC patient prognosis, and what factors explain the discrepancy between SMS transcriptional and protein levels? 08/04/2020

SMS protein alone doesn't impact LSCC patient prognosis, but when combined with GPT, it shows significance. Inconsistency between SMS transcriptional and protein levels may stem from various factors, including the involvement of regulatory elements and different post-transcriptional and post-translational modifications.

How does GPT gene expression, assessed in LSCC tissues and cell lines, correlate with SMS and impact overall survival rates in LSCC patients? 01/06/2019

Analysis of LSCC tissues and cell lines revealed an inverse relationship between GPT and SMS expressions, confirmed through qRT-PCR and TCGA data. GPT protein, primarily located in the epithelium, was down-regulated in LSCC tissues.

How does GPT gene expression in tissues like skeletal muscle, kidney, and liver reflect cancer-related metabolic changes? 05/12/2018

GPT gene expression serves as a marker for cancer-induced metabolic reprogramming in tissues, indicating shifts in energy metabolism and biosynthetic processes.

Customer Reviews (3)

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Reviews
11/13/2021

    Fantastic customer support. They genuinely care about the success of our research.

    10/11/2020

      Top-notch protein quality! It's our go-to for reliable and reproducible results.

      04/06/2018

        Very happy with this lab product!

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