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Recombinant Mouse Grem1 Protein, His-tagged

Cat.No.: Grem1-824M
Product Overview: Recombinant mouse GREM1 (O70326) (Met1-Asp184) was fused with a polyhistidine tag at the C-terminus.
Source: Baculovirus-Insect Cells
Species: Mouse
Tag: His
Predicted N Terminal: Lys 25
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT1, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to inhibit recombinant human BMP4 induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1-7 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
Molecular Mass: The recombinant mouse GREM1 consists of 171 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Grem1 gremlin 1 [ Mus musculus ]
Official Symbol: Grem1
Synonyms: GREM1; gremlin 1; gremlin-1; down-regulated in v-mos-transformed cells; cysteine knot superfamily 1, BMP antagonist 1; down-regulated in Mos-transformed cells protein; ld; Drm; Grem; Cktsf1b1;
Gene ID: 23892
mRNA Refseq: NM_011824
Protein Refseq: NP_035954
MIM:
UniProt ID:
Function: cytokine activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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