| Species : |
Mouse |
| Source : |
Insect Cells |
| Tag : |
His |
| Protein Length : |
1-184 a.a. |
| Description : |
GREM1 is a member of the DAN family. It contains 1 CTCK (C-terminal cystine knot-like) domain and is highly expressed in small intestine, fetal brain and colon. As a cytokine, GREM1 may play an important role during carcinogenesis and metanephric kidney organogenesis, as a BMP antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. It interacts with SLIT1 and SLIT2 in a glycosylation-dependent manner and down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis. GREM1 also is an inhibitor in the TGF beta signaling pathway. It inhibits BMP-2, -4, and -7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. |
| Predicted N Terminal : |
Lys 25 |
| Form : |
Lyophilized from a 0.2μM filtered solution of 20mM Tris, 500mM NaCl, pH7.4,10% gly,0.5mM EDTA, 3mM DTT. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
| Molecular Mass : |
The recombinant protein consists of 171 amino acids and has a calculated molecular mass of 19.7 kDa, migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions. |
| Endotoxin : |
1.0 eu/μg of the protein as determined by the lal |
| Purity : |
>97 % as determined by SDS-PAGE |
| Stability : |
Samples are stable for up to twelve months from date of receipt at -70℃ |
| Storage : |
Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
