Recombinant Mouse GYPA protein, His/Fc-tagged

• Cat.No.: GYPA-396M
• Product Overview: Recombinant Mouse GYPA protein (Met1-Val108), fused to human IgG1 Fc tag at N-terminus and His tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: Glycophorin-A, a member of the glycophorin-A family, is also known as CD235a and Gypa, which is heavily glycosylated on serine and threonine residues at the N-terminal extracellular domain. CD235a,a single-pass type III membrane protein,is the major intrinsic membrane sialoglycoprotein of the erythrocyte. Also,CD235a appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. Furthermore,CD235a may be involved in translocation of SLC4A1 to the plasma membrane.
• Source: HEK293
• Species: Mouse
• Tag: human IgG1 Fc/His
• Form: Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 39.6 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 108
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: GYPA
• Official Symbol: GYPA
• Synonyms: GYPA; glycophorin A; glycophorin-A; GPA; CD235a; AI853584
• Gene ID: 14934
• mRNA Refseq: NM_010369
• Protein Refseq: NP_034499
• UniProt ID: P14220