Recombinant Mouse H1f8 Protein, Myc/DDK-tagged

• Cat.No.: H1f8-3344M
• Product Overview: Purified recombinant protein of mouse full-length H1 histone family, member O, oocyte-specific (H1foo), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein encoded is a replication-independent histone that is a member of the histone H1 family. This gene contains introns, unlike most histone genes and the encoded protein is expressed only in oocytes.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 32.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: H1f8 H1.8 linker histone [ Mus musculus (house mouse) ]
• Official Symbol: H1f8
• Synonyms: H1f8; H1.8 linker histone; H1; H1f; H1-8; H1.8; H1fo; H1oo; H1foo; C86609; histone H1.8; H1 histone family, member O, oocyte-specific; histone H1oo; oocyte-specific histone H1; oocyte-specific linker histone H1
• Gene ID: 171506
• mRNA Refseq: NM_138311
• Protein Refseq: NP_612184
• UniProt ID: Q8VIK3