"IFNB1" Related Products

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Recombinant Mouse IFNB1 Protein, Fc-tagged

Cat.No.: IFNB1-892M
Product Overview: Recombinant extracellular domain of mouse IFNB1 (P01575) (Met 1-Asn 182) was fused with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Mouse
Tag: Fc
Predicted N Terminal: Ile 22
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 10-50 pg/mL.
Molecular Mass: The secreted recombinant mouse IFNB1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 402 amino acids and has a calculated molecular mass of 46.7 kDa. As a result of glycosylation, the apparent molecular mass of rmIFNB1/Fc monomer is approximately 58 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >92 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Ifnb1 interferon beta 1, fibroblast [ Mus musculus ]
Official Symbol: IFNB1
Synonyms: IFNB1; interferon beta 1, fibroblast; interferon beta; Ifb; IFNB; IFN-beta;
Gene ID: 15977
mRNA Refseq: NM_010510
Protein Refseq: NP_034640
MIM:
UniProt ID:
Pathway: Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved
Function: cytokine activity; cytokine receptor binding; transcription corepressor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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