Recombinant Mouse IFNG Protein, His-tagged

Cat.No.: IFNG-893M
Product Overview: Recombinant mouse IFNG (P01580) (Met 1-Cys 155) was expressed, fused with a polyhistidine tag at the C-terminus.
Source: HEK293
Species: Mouse
Tag: His
Predicted N Terminal: His 23
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 0.2-1 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His at 10 μg/ml (100 μl/wel
Molecular Mass: The recombinant mouse IFNG comprises 144 amino acids and has a calculated molecular mass of 17 kDa. As a result of glycosylation, the apparent molecular mass of rn IFNG is approximately 21 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >75 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Ifng interferon gamma [ Mus musculus ]
Official Symbol: IFNG
Synonyms: IFNG; interferon gamma; IFN-gamma; gamma interferon; Ifg; IFN-g;
Gene ID: 15978
mRNA Refseq: NM_008337
Protein Refseq: NP_032363
MIM:
UniProt ID:
Pathway: African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved
Function: cytokine activity; interferon-gamma receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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