Recombinant Mouse Ift27 Protein, Myc/DDK-tagged

• Cat.No.: Ift27-3484M
• Product Overview: Purified recombinant protein of mouse full-length intraflagellar transport 27 (Ift27), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Small GTPase-like component of the intraflagellar transport (IFT) complex B that promotes the exit of the BBSome complex from cilia via its interaction with ARL6. Not involved in entry of the BBSome complex into cilium. Prevents aggregation of GTP-free ARL6. Required for hedgehog signaling. Forms a subcomplex within the IFT complex B with IFT25. Its role in intraflagellar transport is mainly seen in tissues rich in ciliated cells such as kidney and testis. Essential for male fertility, spermiogenesis and sperm flagella formation. Plays a role in the early development of the kidney. May be involved in the regulation of ureteric bud initiation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 20.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ift27 intraflagellar transport 27 [ Mus musculus (house mouse) ]
• Official Symbol: Ift27
• Synonyms: IFT27; intraflagellar transport 27 homolog (Chlamydomonas); intraflagellar transport protein 27 homolog; rab-like protein 4; RAB, member of RAS oncogene family-like 4; Rabl4; 2600013G09Rik
• Gene ID: 67042
• mRNA Refseq: NM_025931
• Protein Refseq: NP_080207
• UniProt ID: Q9D0P8