Recombinant Mouse IL12A & IL12B Heterotrimer Protein (Met1-Ala215 & Met1-Ser335), HlgG1 Fc-tagged IL12-1031M

Recombinant Mouse IL12A & IL12B Heterotrimer Protein (Met1-Ala215 & Met1-Ser335), HlgG1 Fc-tagged

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Recombinant Mouse IL12A & IL12B Heterotrimer Protein (Met1-Ala215 & Met1-Ser335), HlgG1 Fc-tagged Online Inquiry

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Cat.No. : IL12-1031M Optional Service: Optional requirements on this protein
Product Overview : Recombinant Mouse IL12A & IL12B Heterotrimer Protein (P43431)(Met1-Ala215) & (P43432)(Met1-Ser335) was produced by HEK293 Cells expression system. This protein was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid. The two plasmids were co-expressed and the heterodimer was purified.
Source : HEK293
Species : Mouse
Tag : HlgG1 Fc
Predicted N Terminal : Arg 23 & Met 23
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Immobilized mouse mIL12A+mIL12B at 10 μg/ml (100 μl/well) can bind biotinylated human IL12RB1-His, The EC50 of biotinylated human IL12RB1-His is 38.7-90.3 ng/ml.
2. Measured in a cell proliferation assay using Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.2-0.8 ng/mL.
Molecular Mass : The recombinant mouse IL12 heterodimer of IL12A/IL12B, both was Fc chimera, comprises 988 (434 + 554) amino acids and has a calculated molecular mass of 111.5 (48.7 + 62.8) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 55 & 75 kDa respectively in SDS-PAGE under reducing conditions.
Protein length : Met1-Ala215 & Met1-Ser335
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 85 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : Il12a interleukin 12a [ Mus musculus ] & Il12b interleukin 12b [ Mus musculus ]
Official Symbol : IL12A & IL12B
Synonyms : IL12A; CLMF p35; p35; Ll12a; Il-12a; IL-12p35; MGC151228; MGC151232 & IL12B; CLMF p40; IL-12 p40; IL-12 subunit p40; IL-23 subunit p40; p40; Il-12b; Il12p40; Il-12p40;
Gene ID : 16159 & 16160
mRNA Refseq : NM_001159424 & NM_008352
Protein Refseq : NP_001152896 & NP_032378
UniProt ID : P43431 & P43432
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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