Recombinant Mouse Il1r2 Protein, His-tagged

• Cat.No.: Il1r2-1670M
• Product Overview: Recombinant Mouse Il1r2 Protein (Phe14-Pro247) with N-His tag was expressed in E. coli.

Specification

• Description: Enables interleukin-1 binding activity and interleukin-1 receptor activity. Acts upstream of or within negative regulation of gene expression and negative regulation of interleukin-1-mediated signaling pathway. Located in cytoplasm. Is expressed in several structures, including dorsal aorta; gut; pituitary gland; reproductive system; and thymus. Human ortholog(s) of this gene implicated in aggressive periodontitis. Orthologous to human IL1R2 (interleukin 1 receptor type 2).
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.9 kDa
  Accurate Molecular Mass: 30 kDa
• Protein length: Phe14-Pro247
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Il1r2 interleukin 1 receptor, type II [ Mus musculus (house mouse) ]
• Official Symbol: Il1r2
• Synonyms: IL1R2; interleukin 1 receptor, type II; interleukin-1 receptor type 2; IL-1R-2; IL-1RT2; IL-1RT-2; IL-1R-beta; IL-1 type II receptor; IL-1 receptor beta chain; interleukin-1 receptor beta; interleukin-1 receptor type II; CD121 antigen-like family member B; CD121b; Il1r-2
• Gene ID: 16178
• mRNA Refseq: NM_010555
• Protein Refseq: NP_034685
• UniProt ID: P27931

Reviews

10/10/2022

High specificity.

09/12/2020

Good for flow cytometry.

09/20/2016

Suitable for co-immunoprecipitation.

Q&As

What are the functional implications of IL1R2's intracellular domain, and how can engineered receptor constructs shed light on its contributions?

The intracellular domain of IL1R2 has functional significance; engineered constructs clarify its role.

How does IL1R2's glycosylation affect its interaction with IL-1 ligands, and how can glycoproteomics methods elucidate these glycosylation sites?

Glycosylation of IL1R2 affects its IL-1 ligand interaction, and glycoproteomics methods can reveal critical glycosylation sites.

In what ways can engineered IL1R2 variants with altered domains provide insights into its molecular mechanisms and potential therapeutic applications?

Engineered IL1R2 variants offer insights into its mechanisms and therapeutic potential.

In what ways does IL1R2's presence influence the downstream signaling of IL-1 ligands through its competition with IL1R1, and how can genetic manipulation techniques help unravel this interplay?

IL1R2's presence impacts IL-1 ligand signaling through competition with IL1R1; genetic manipulations unveil this interaction.

How do post-translational modifications, particularly ubiquitination, influence IL1R2's stability and turnover?

Post-translational modifications, including ubiquitination, influence IL1R2's stability.

How does IL1R2's expression respond to inflammatory cues, and what role do transcription factors play in its regulatory network?

IL1R2 expression responds to inflammation; transcription factors play a role in its regulation.

What insights can super-resolution microscopy offer regarding IL1R2's intracellular trafficking and subcellular localization dynamics?

Super-resolution microscopy helps understand IL1R2's trafficking and subcellular dynamics in detail.

What specific structural features of IL1R2 enable it to function as a decoy receptor, and how can X-ray crystallography provide a detailed understanding of its ligand binding mechanisms?

Specific structural elements of IL1R2 contribute to its decoy role; X-ray crystallography provides insights into ligand binding.

How does the shedding of IL1R2's ectodomain contribute to its role as a soluble decoy receptor, and which enzymes are involved in this process?

Ectodomain shedding contributes to IL1R2's soluble decoy role; specific enzymes are involved.

What significance does IL1R2's interaction with IL-1 receptor accessory proteins hold in terms of its decoy function?

IL1R2's interaction with accessory proteins is significant for its decoy function.