| Cat.No. : |
IL23A&IL12B-336M
Optional Service: Optional requirements on this protein
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| Product Overview : |
Recombinant Mouse IL23A & IL12B Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14 (IL23A) & NP_032378 (IL12B)). Predicted N-terminus: His (IL23A) & Met 23 (IL12B). Mouse IL-23 alpha & IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 20.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The predicted N-terminus is His (IL23A) & Met 23 (IL12B). The reducing (R) protein migrates as 21 kDa (IL-23 alpha) and 45-50 kDa (IL-12 beta) respectively due to glycosylation. |
| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization. |
| Bio-activity : |
Recombinant Mouse IL23A & IL12B Heterodimer Protein, His-tagged at 10 μg/mL (100 μL/well) can bind Human IL23R with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Recombinant Mouse IL23A & IL12B Heterodimer Protein, His-tagged stimulates secretion of IL-17 by mouse spleen cells. The ED50 for this effect is 0.008-0.0134ng/ml (Routinely tested). |
| Molecular Mass : |
20.5 kDa (IL23A) & 35.8 kDa (IL12B) |
| Protein length : |
Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) |
| Endotoxin : |
Less than 1.0 EU per μg by the LAL method. |
| Purity : |
>90% as determined by SDS-PAGE. |
| Notes : |
Please avoid repeated freeze-thaw cycles. |
| Stability : |
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution. |
| Storage : |
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
| Figure : |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
