Active Recombinant Mouse IL23A & IL12B Heterodimer Protein, His-tagged

• Cat.No.: IL23A&IL12B-336M
• Product Overview: Recombinant Mouse IL23A & IL12B Heterodimer Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B) (Accession # Q9EQ14 (IL23A) & NP_032378 (IL12B)). Predicted N-terminus: His (IL23A) & Met 23 (IL12B). Mouse IL-23 alpha & IL-12 beta Heterodimer Protein, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 20.5 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The predicted N-terminus is His (IL23A) & Met 23 (IL12B). The reducing (R) protein migrates as 21 kDa (IL-23 alpha) and 45-50 kDa (IL-12 beta) respectively due to glycosylation.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Mouse IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free at 2 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 0.02-0.313 μg/mL.
• Molecular Mass: The protein has a calculated MW of 20.5 kDa (IL-23A) and 35.8 kDa (IL-12B). Subunit IL-23A is fused with a polyhistidine tag at the N-terminus and subunit IL-12B contains no tag. The reducing (R) protein migrates as 19 kDa (IL-23A) and 40-44 kDa (IL-12B) respectively due to glycosylation.
• Protein Length: Val 22 - Ala 196 (IL23A) & Met 23 - Ser 335 (IL12B)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Il23a interleukin 23, alpha subunit p19 [ Mus musculus ]
• Official Symbol: IL23A
• Synonyms: IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin-23 subunit p19; p19; IL-23
• Gene ID: 83430
• mRNA Refseq: NM_031252
• Protein Refseq: NP_112542