Recombinant Human Il12B&Il23A Protein, C-His-tagged

• Cat.No.: IL23A&IL12B-335H
• Product Overview: Recombinant Human Il12B&Il23A Protein(Q9NPF7&P29460)(Arg20-Pro189&Ile23-Ser328) is expressed from HEK293 with His tag at the C-terminus of IL-23 alpha.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 20-189 aa&23-328 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human IL-23R, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 46.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19.77 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Due to glycosylation, the protein migrates to 23 kDa (IL-23 alpha) and 40-50 kDa (IL-12 beta) based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL23A interleukin 23, alpha subunit p19 [ Homo sapiens ]
• Official Symbol: Il23A
• Synonyms: IL23A; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; IL 23; IL 23A; IL23P19; interleukin six; G CSF related factor; P19; SGRF; IL-23-A; IL-23p19; IL-23 subunit alpha; interleukin 23 p19 subunit; interleukin-23 subunit p19; JKA3 induced upon T-cell activation; interleukin-six, G-CSF related factor; IL-23; IL-23A; MGC79388
• Gene ID: 51561
• mRNA Refseq: NM_016584
• Protein Refseq: NP_057668
• MIM: 605580
• UniProt ID: Q9NPF7
• Gene Name 2: IL12B interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) [ Homo sapiens ]
• Official Symbol 2: Il12B
• Synonyms 2: IL12B; interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40); NKSF2; interleukin-12 subunit beta; CLMF; CLMF2; cytotoxic lymphocyte maturation factor 2; p40; IL 12B; IL12; subunit p40; interleukin 12; interleukin 12 beta chain; natural killer cell stimulatory factor; 40 kD subunit; natural killer cell stimulatory factor 2; NKSF; CLMF p40; IL-12 subunit p40; IL12, subunit p40; interleukin 12, p40; interleukin-12 beta chain; NK cell stimulatory factor chain 2; cytotoxic lymphocyte maturation factor 40 kDa subunit; natural killer cell stimulatory factor, 40 kD subunit; IL-12B
• Gene ID 2: 3593
• mRNA Refseq 2: NM_002187
• Protein Refseq 2: NP_002178
• MIM 2: 161561
• UniProt ID 2: P29460