Active Recombinant Mouse Il6 protein, His-tagged, Low Endotoxin

• Cat.No.: Il6-490M
• Product Overview: Recombinant Mouse Il6 protein(P08505)(25-211 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 25-211 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Mouse IL-6 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-6, His Tag with an affinity constant of 0.15 μM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 22.83 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Il6 interleukin 6 [ Mus musculus ]
• Official Symbol: Il6
• Synonyms: IL6; interleukin 6; interleukin-6; interleukin HP-1; B-cell hybridoma growth factor; Il-6
• Gene ID: 16193
• mRNA Refseq: NM_031168
• Protein Refseq: NP_112445