Recombinant Mouse Inpp5e Protein, Myc/DDK-tagged

• Cat.No.: Inpp5e-3548M
• Product Overview: Purified recombinant protein of mouse full-length inositol polyphosphate-5-phosphatase E (Inpp5e), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Specific for lipid substrates, inactive towards water soluble inositol phosphates. Specific for lipid substrates, inactive towards water soluble inositol phosphates. Plays an essential role in the primary cilium by controlling ciliary growth and phosphoinositide 3-kinase (PI3K) signaling and stability.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 71.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Inpp5e inositol polyphosphate-5-phosphatase E [ Mus musculus (house mouse) ]
• Official Symbol: Inpp5e
• Synonyms: INPP5E; inositol polyphosphate-5-phosphatase E; 72 kDa inositol polyphosphate 5-phosphatase; inositol polyphosphate-5-phosphatase, 72 kDa; phosphatidylinositol 4,5-bisphosphate 5-phosphatase; phosphatidylinositol-4,5-bisphosphate 5-phosphatase; phosphatidylinositol polyphosphate 5-phosphatase type IV; 72kDa; mKIAA0123; 1200002L24Rik
• Gene ID: 64436
• mRNA Refseq: NM_033134
• Protein Refseq: NP_149125
• UniProt ID: Q9JII1

Reviews

06/18/2020

We felt well-supported throughout our usage of the product, thanks to the dedicated customer service.

05/05/2020

Its ease of use allowed us to focus more on data analysis and interpretation, rather than technical challenges.

04/17/2019

The product's ability to accelerate research progress made it invaluable for time-critical projects.

Q&As

Is there an involvement of AKT in the interaction between INPP5E and AURKA?

Yes, partly through an AKT-dependent mechanism, the interaction between INPP5E and AURKA affects the downregulation of AURKA.

What is the role of INPP5E in ciliary biology?

INPP5E plays a significant role in ciliary biology and is associated with ciliopathies like Joubert and MORM syndromes.

How does the increased 5-phosphatase activity of INPP5E influence AURKA?

INPP5E's increased 5-phosphatase activity promotes transcriptional downregulation of AURKA.

What is the interaction between INPP5E and AURKA?

INPP5E interacts with AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly.

What does this interaction between AURKA and INPP5E establish?

This interaction establishes the first direct link between AURKA and phosphoinositide signaling.

How does the interaction between INPP5E and AURKA affect primary cilia stability?

The interaction between INPP5E and AURKA is crucial for maintaining the stability of primary cilia.

What is the impact of AURKA phosphorylation on INPP5E?

AURKA phosphorylates INPP5E, enhancing its 5-phosphatase activity.