Recombinant Human INPP5E Protein, MYC/DDK-tagged

• Cat.No.: INPP5E-2396H
• Product Overview: Recombinant Human INPP5E protein, fused to MYC/DDK-tagged at C-terminus, was expressed in HEK293.

Specification

• Description: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 70 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: INPP5E inositol polyphosphate-5-phosphatase, 72 kDa [ Homo sapiens ]
• Official Symbol: INPP5E
• Synonyms: CORS1; CPD4; JBTS1; MORMS; PPI5PIV
• Gene ID: 56623
• mRNA Refseq: NM_019892
• Protein Refseq: NP_063945
• MIM: 613037
• UniProt ID: Q9NRR6

Reviews

06/18/2020

We felt well-supported throughout our usage of the product, thanks to the dedicated customer service.

05/05/2020

Its ease of use allowed us to focus more on data analysis and interpretation, rather than technical challenges.

04/17/2019

The product's ability to accelerate research progress made it invaluable for time-critical projects.

Q&As

Is there an involvement of AKT in the interaction between INPP5E and AURKA?

Yes, partly through an AKT-dependent mechanism, the interaction between INPP5E and AURKA affects the downregulation of AURKA.

What is the role of INPP5E in ciliary biology?

INPP5E plays a significant role in ciliary biology and is associated with ciliopathies like Joubert and MORM syndromes.

How does the increased 5-phosphatase activity of INPP5E influence AURKA?

INPP5E's increased 5-phosphatase activity promotes transcriptional downregulation of AURKA.

What is the interaction between INPP5E and AURKA?

INPP5E interacts with AURKA, a centrosomal kinase that regulates mitosis and ciliary disassembly.

What does this interaction between AURKA and INPP5E establish?

This interaction establishes the first direct link between AURKA and phosphoinositide signaling.

How does the interaction between INPP5E and AURKA affect primary cilia stability?

The interaction between INPP5E and AURKA is crucial for maintaining the stability of primary cilia.

What is the impact of AURKA phosphorylation on INPP5E?

AURKA phosphorylates INPP5E, enhancing its 5-phosphatase activity.