Recombinant Mouse IRF3 Protein (1-419 aa), His-Myc-tagged

• Cat.No.: IRF3-2146M
• Product Overview: Recombinant Mouse IRF3 Protein (1-419 aa) is produced by Yeast expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Immunology. Protein Description: Full Length.

Specification

• Description: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
• Source: Yeast
• Species: Mouse
• Tag: His-Myc
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 50.9 kDa
• Protein length: 1-419 aa
• AA Sequence: METPKPRILPWLVSQLDLGQLEGVA WLDESRTRFRIPWKHGLRQDAQMAD FGIFQAWAEASGAYTPGKDKPDVST WKRNFRSALNRKEVLRLAADNSKDP YDPHKVYEFVTPGARDFVHLGASPD TNGKSSLPHSQENLPKLFDGLILGP LKDEGSSDLAIVSDPSQQLPSPNVN NFLNPAPQENPLKQLLAEEQWEFEV TAFYRGRQVFQQTLFCPGGLRLVGS TADMTLPWQPVTLPDPEGFLTDKLV KEYVGQVLKGLGNGLALWQAGQCLW AQRLGHSHAFWALGEELLPDSGRGP DGEVHKDKDGAVFDLRPFVADLIAF MEGSGHSPRYTLWFCMGEMWPQDQP WVKRLVMVKVVPTCLKELLEMAREG GASSLKTVDLHISNSQPISLTSDQY KAYLQDLVEDMDFQATGNI
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: Irf3 interferon regulatory factor 3 [ Mus musculus ]
• Official Symbol: IRF3
• Synonyms: IRF3; interferon regulatory factor 3; IRF-3; C920001K05Rik; MGC91046
• Gene ID: 54131
• mRNA Refseq: NM_016849
• Protein Refseq: NP_058545
• UniProt ID: P70671