Recombinant Mouse ITGAV&ITGB3 protein, His-Avi-tagged, Biotinylated

• Cat.No.: ITGAV&ITGB3-1427M
• Product Overview: Biotinylated Recombinant Mouse ITGAV&ITGB3 protein(P43406(ITGAV)&O54890(ITGB3))(Phe31-Val988(ITGAV)& Glu26-Asp717(ITGB3)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Phe31-Val988(ITGAV)& Glu26-Asp717(ITGB3)
• Tag: C-His-Avi
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human Vitronectin, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse ITGAV&ITGB3, His Tag with the EC50 of 3.2μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 108.77 kDa(ITGAV)&80.84 kDa(ITGB3). Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: Itgav integrin alpha V [ Mus musculus ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; integrin alpha V; integrin alpha-V; alphav-integrin; vitronectin receptor subunit alpha; vitronectin receptor alpha polypeptide (VNRA); CD51; 1110004F14Rik; 2610028E01Rik; D430040G12Rik
• Gene ID: 16410
• mRNA Refseq: NM_008402
• Protein Refseq: NP_032428
• Gene Name 2: Itgb3 integrin beta 3 [ Mus musculus ]
• Official Symbol 2: ITGB3
• Synonyms 2: ITGB3; integrin beta 3; integrin beta-3; GPIIIa; platelet gpIIIa; platelet membrane glycoprotein IIIa; CD61; GP3A; INGRB3; MGC159221; MGC159223
• Gene ID 2: 16416
• mRNA Refseq 2: NM_016780
• Protein Refseq 2: NP_058060