Recombinant Mouse ITGB6 Protein

• Cat.No.: Itgb6-460M
• Product Overview: Recombinant extracellular domain (Met 1-Val988) of mouse ITGAV (NP_032428.2) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Pro708) of mouse ITGB6 (NP_067334.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse ITGAV&ITGB6 heterodimer was purified.

Specification

• Description: Integrin alpha-5, also known as ITGA5, is a single-pass type I membrane protein which belongs to the integrin alpha chain family. ITGA5 contains 7 FG-GAP repeats. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. ITGAV&ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of ITGAV&ITGB6 via clathrin-mediated end centigradeytosis promotes carcinoma cell invasion.
• Source: HEK293
• Species: Mouse
• Predicted N Terminal: Phe 31 & Gly 22
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of mouse ITGAV&ITGB6 comprises 1728 (1000+728) amino acids and has a calculated molecular mass of 190.4 (110.7+79.7) KDa.
• Protein length: Met 1-Val988
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > (32.4+62.5) % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Itgb6 integrin beta 6 [ Mus musculus ]
• Official Symbol: Itgb6
• Synonyms: ITGB6; integrin beta 6; integrin beta-6; 2210409C20Rik; 4831415H04Rik
• Gene ID: 16420
• mRNA Refseq: NM_001159564
• Protein Refseq: NP_001153036