Active Recombinant Mouse Lifr Protein, His-tagged

• Cat.No.: Lifr-021M
• Product Overview: Recombinant mouse LIFR alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
• Source: Insect cells
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤ 1μg/mL with Mouse LIF.
• Molecular Mass: 89.6 kDa
• AA Sequence: LKRGVQDLKCTTNNMRVWDCTWPAP LGVSPGTVKDICIKDRFHSCHPLET TNVKIPALSPGDHEVTINYLNGFQS KFTLNEKDVSLIPETPEILDLSADF FTSSLLLKWNDRGSALPHPSNATWE IKVLQNPRTEPVALVLLNTMLSGKD TVQHWNWTSDLPLQCATHSVSIRWH IDSPHFSGYKEWSDWSPLKNISWIR NTETNVFPQDKVVLAGSNMTICCMS PTKVLSGQIGNTLRPLIHLYGQTVA IHILNIPVSENSGTNIIFITDDDVY GTVVFAGYPPDVPQKLSCETHDLKE IICSWNPGRITGLVGPRNTEYTLFE SISGKSAVFHRIEGLTNETYRLGVQ MHPGQEIHNFTLTGRNPLGQAQSAV VINVTERVAPHDPTSLKVKDINSTV VTFSWYLPGNFTKINLLCQIEICKA NSKKEVRNATIRGAEDSTYHVAVDK LNPYTAYTFRVRCSSKTFWKWSRWS DEKRHLTTEATPSKGPDTWREWSSD GKNLIVYWKPLPINEANGKILSYNV SCSLNEETQSVLEIFDPQHRAEIQL SKNDYIISVVARNSAGSSPPSKIAS MEIPNDDITVEQAVGLGNRIFLTWR HDPNMTCDYVIKWCNSSRSEPCLLD WRKVPSNSTETVIESDQFQPGVRYN FYLYGCTNQGYQLLRSIIGYVEELA PIVAPNFTVEDTSADSILVKWDDIP VEELRGFLRGYLFYFQKGERDTPKT RSLEPHHSDIKLKNITDISQKTLRI ADLQGKTSYHLVLRAYTHGGLGPEK SMFVVTKENS
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -100 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Lifr LIF receptor alpha [ Mus musculus (house mouse) ]
• Official Symbol: Lifr
• Synonyms: Lifr; LIF receptor alpha; LIF; AW061234; A230075M04Rik; leukemia inhibitory factor receptor; D-factor/LIF receptor; LIF-R; leukemia inhibitory factor receptor alpha chain; soluble differentiation-stimulating factor receptor
• Gene ID: 16880
• mRNA Refseq: NM_013584
• Protein Refseq: NP_038612
• UniProt ID: P42703