Recombinant Mouse Lifr Protein, His-tagged

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Recombinant Mouse Lifr Protein, His-tagged

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Cat.No. : Lifr-021M
Product Overview : Recombinant mouse LIFR alpha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description : Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.
Source : Insect cells
Species : Mouse
Tag : His
Form : Liquid
Bio-activity : Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤ 1μg/mL with Mouse LIF.
Molecular Mass : 89.6 kDa
AA Sequence : <ADP>LKRGV QDLKCTTNNM RVWDCTWPAP LGVSPGTVKD ICIKDRFHSC HPLETTNVKI PALSPGDHEV TINYLNGFQS KFTLNEKDVS LIPETPEILD LSADFFTSSL LLKWNDRGSA LPHPSNATWE IKVLQNPRTE PVALVLLNTM LSGKDTVQHW NWTSDLPLQC ATHSVSIRWH IDSPHFSGYK EWSDWSPLKN ISWIRNTETN VFPQDKVVLA GSNMTICCMS PTKVLSGQIG NTLRPLIHLY GQTVAIHILN IPVSENSGTN IIFITDDDVY GTVVFAGYPP DVPQKLSCET HDLKEIICSW NPGRITGLVG PRNTEYTLFE SISGKSAVFH RIEGLTNETY RLGVQMHPGQ EIHNFTLTGR NPLGQAQSAV VINVTERVAP HDPTSLKVKD INSTVVTFSW YLPGNFTKIN LLCQIEICKA NSKKEVRNAT IRGAEDSTYH VAVDKLNPYT AYTFRVRCSS KTFWKWSRWS DEKRHLTTEA TPSKGPDTWR EWSSDGKNLI VYWKPLPINE ANGKILSYNV SCSLNEETQS VLEIFDPQHR AEIQLSKNDY IISVVARNSA GSSPPSKIAS MEIPNDDITV EQAVGLGNRI FLTWRHDPNM TCDYVIKWCN SSRSEPCLLD WRKVPSNSTE TVIESDQFQP GVRYNFYLYG CTNQGYQLLR SIIGYVEELA PIVAPNFTVE DTSADSILVK WDDIPVEELR GFLRGYLFYF QKGERDTPKT RSLEPHHSDI KLKNITDISQ KTLRIADLQG KTSYHLVLRA YTHGGLGPEK SMFVVTKENS <HHHHHH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Bioactivity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -100 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by Absorbance at 280nm)
Storage Buffer : Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
SDS-PAGE :

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Biological Activity :

Mouse LIFR alpha inhibits human LIF induced cell proliferation in the TF-1 human erythroleukemic cells. The ED50 range is ≤ 1 μg/mL.

Gene Name : Lifr LIF receptor alpha [ Mus musculus (house mouse) ]
Official Symbol : Lifr
Synonyms : Lifr; LIF receptor alpha; LIF; AW061234; A230075M04Rik; leukemia inhibitory factor receptor; D-factor/LIF receptor; LIF-R; leukemia inhibitory factor receptor alpha chain; soluble differentiation-stimulating factor receptor
Gene ID : 16880
mRNA Refseq : NM_013584
Protein Refseq : NP_038612
UniProt ID : P42703

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