|Product Overview:||Recombinant Mouse Pcsk9(Gln26-Gln776) fused with His tag at C-terminal was expressed in NS0.|
|Description:||Lysyl Oxidase Homolog 2 (lysyl oxidase-like protein 2, LOXL2) is a member of lysyl oxidase-like (LOXL) gene family which includes LOXL1 through LOXL4. These enzymes are secreted copper-binding amine oxidases that oxidize primary amine substrates to aldehydes. The N-terminal region of LOXL2 contains four scavenger receptor cysteine-rich (SRCR) domains, and the C-terminal region is a catalytic domain similar to other lysyl oxidases. The catalytic domain contains conserved residues required for copper binding and formation of a lysyl tyrosylquinone co-factor. It has been shown that LOXL2 promotes cell migration and tumor cell invasiveness. Elevated expression of LOXL2 is also associated with cancer progression in various tumors and carcinoma cell lines, which makes it a potential marker for prognosis of cancer. LOXL2 is expressed in many tissues, with elevated levels in reproductive tissues such as placenta, uterus, and prostate. Activity is routinely determined using benzylamine as a substrate, although significantly higher activity is detected when activity is quantified using lysine as the substrate.|
|Predicted N Terminal:||No results obtained. Gln26 inferred from enzymatic pyroglutamate treatment revealing Tyr27|
|Form:||Supplied as a 0.2 μm filtered solution in MES and NaCl.|
|Bio-activity:||Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine. The specific activity is >4.5 pmol/min/μg.|
|Molecular Mass:||Predicted Molecular Mass: 85 kDa
SDS-PAGE: 93-111 kDa, reducing conditions
|Endotoxin:||<1.0 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
|Gene Name:||Loxl2 lysyl oxidase-like 2 [ Mus musculus ]|
|Synonyms:||LOXL2; lysyl oxidase-like 2; lysyl oxidase homolog 2; lysyl oxidase-like protein 2; 1110004B06Rik; 4930526G11Rik; 9430067E15Rik; MGC102220;|