Recombinant Mouse Lysophospholipase 1, His-tagged

• Cat.No.: Lypla1-2813M
• Product Overview: Recombinant mouse LYPLA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. MW = 26.8 kDa (250aa), confirmed by MALDI-TOF.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: LYPLA1 is lysophospholipase that acts on biological membranes to regulate the multifunctional lysophospholipids. This protein hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS and also has depalmitoylating activity.
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MCGNNMSAPM PAVVPAARKA TAAVIFLHGL GDTGHGWAEA FAGIKSPHIK YICPHAPVMP VTLNMNMAMP SWFDIVGLSP DSQEDESGIK QAAETVKALI DQEVKNGIPS NRIILGGFSQ GGALSLYTAL TTQQKLAGVT ALSCWLPLRA SFSQGPINSA NRDISVLQCH GDCDPLVPLM FGSLTVERLK ALINPANVTF KIYEGMMHSS CQQEMMDVKH FIDKLLPPID
• Purity: > 90% by SDS – PAGE.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl,1mM DTT.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: Lypla1 lysophospholipase 1 [ Mus musculus ]
• Synonyms: Lypla1; lysophospholipase 1; Pla1a; acyl-protein thioesterase 1; APT-1; LPL-I; lysoPLA I; phospholipase 1a; OTTMUSP00000024030; OTTMUSP00000024032; OTTMUSP00000024033; lysophopholipase 1; lysophospholipase I; EC 3.1.1.5
• Gene ID: 18777
• mRNA Refseq: NM_008866
• Protein Refseq: NP_032892
• UniProt ID: P97823
• Chromosome Location: 1 A1; 1
• Pathway: Glycerophospholipid metabolism
• Function: hydrolase activity; lysophospholipase activity