Recombinant Mouse Map2k4 Protein, Myc/DDK-tagged

• Cat.No.: Map2k4-3923M
• Product Overview: Purified recombinant protein of mouse full-length mitogen activated protein kinase kinase 4 (cDNA clone MGC:36532 IMAGE:3962163), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 32.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Map2k4 mitogen-activated protein kinase kinase 4 [ Mus musculus (house mouse) ]
• Official Symbol: Map2k4
• Synonyms: Map2k4; mitogen-activated protein kinase kinase 4; Sek; Ser; JNKK; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4; MAPKK 4; dual specificity mitogen-activated protein kinase kinase 4; C-JUN N-terminal kinase kinase 1; JNK kinase 1; JNK-activating kinase 1; MAP kinase kinase 4; MAPK/ERK kinase 4; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; mitogen activated protein kinase kinase 4, variant 1; EC 2.7.12.2
• Gene ID: 26398
• mRNA Refseq: NM_009157
• Protein Refseq: NP_033183
• UniProt ID: P47809