Recombinant Mouse Mapk9 Protein, Myc/DDK-tagged

• Cat.No.: Mapk9-3948M
• Product Overview: Purified recombinant protein of mouse full-length mitogen-activated protein kinase 9 (Mapk9), transcript variant alpha1, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteosomal degradation
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 48 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mapk9 mitogen-activated protein kinase 9 [ Mus musculus (house mouse) ]
• Official Symbol: Mapk9
• Synonyms: MAPK9; mitogen-activated protein kinase 9; MAPK 9; Jun kinase; MAP kinase 9; JNK/SAPK alpha; c-Jun N-terminal kinase 2; mitogen activated protein kinase 9; protein kinase, mitogen-activated 9; stress-activated protein kinase JNK2; JNK2; Prkm9; AI851083; p54aSAPK
• Gene ID: 26420
• mRNA Refseq: NM_001163672
• Protein Refseq: NP_001157144
• UniProt ID: Q9WTU6