Recombinant Mouse Mapt protein, His-tagged

• Cat.No.: Mapt-19M
• Product Overview: Recombinant Mouse Mapt(Ala92~Val400) fused with 10-his tag at C-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: Predicted Molecular Mass: 36.0kDa;
  Accurate Molecular Mass: 44kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Mapt microtubule-associated protein tau [ Mus musculus ]
• Official Symbol: Mapt
• Synonyms: MAPT; microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; Tau; Mtapt; AI413597; AW045860
• Gene ID: 17762
• mRNA Refseq: NM_001038609
• Protein Refseq: NP_001033698
• UniProt ID: P10637

Reviews

07/22/2022

Western-blot experiments using MAPT as a target have shown highly reproducible results.

08/08/2020

This stability of MAPT is very good and can maintain its original properties even after multiple uses.

09/15/2019

MAPT's purity and consistency make it a top choice for Western-blot experiments.

Q&As

How to assess the degree of abnormal aggregation of MAPT protein?

The degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.

What is the relationship between MAPT protein and nerve cytoskeleton?

The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.

What are the interactions of the MAPT protein with other proteins in the nervous system?

MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.

Is there a mechanism that regulates the expression of MAPT protein?

Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.

What are the future directions for MAPT protein research?

Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.

How about the applications of MAPT protein in clinical diagnosis?

The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.