Active Recombinant Mouse Mfge8 protein, His-tagged

• Cat.No.: Mfge8-408M
• Product Overview: Recombinant Mouse Mfge8 protein(Ala23-Cys463), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 23-463 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 25.33-101.30 ng/mL, corresponding to a specific activity of 9.87×10^3~3.95×10^4 units/mg.
• Molecular Mass: 49.84 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Mfge8 milk fat globule-EGF factor 8 protein [ Mus musculus ]
• Official Symbol: Mfge8
• Synonyms: MFGE8; milk fat globule-EGF factor 8 protein; lactadherin; MP47; EGF/factor VIII; sperm surface protein SP47; milk fat globule glycoprotein; P47; Mfgm; SED1; MFG-E8; AA408458; AI325141
• Gene ID: 17304
• mRNA Refseq: NM_001045489
• Protein Refseq: NP_001038954