Recombinant Mouse Mgme1 Protein, Myc/DDK-tagged

• Cat.No.: Mgme1-4061M
• Product Overview: Purified recombinant protein of mouse full-length mitochondrial genome maintenance exonuclease 1 (Mgme1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair. Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 38.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mgme1 mitochondrial genome maintenance exonuclease 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mgme1
• Synonyms: Mgme1; mitochondrial genome maintenance exonuclease 1; AI426476; 8430406I07Rik; mitochondrial genome maintenance exonuclease 1; mitochondrial genome maintainance exonuclease 1
• Gene ID: 74528
• mRNA Refseq: NM_028984
• Protein Refseq: NP_083260
• UniProt ID: Q9CXC3