Recombinant Mouse Mief1 Protein, Myc/DDK-tagged

• Cat.No.: Mief1-4071M
• Product Overview: Purified recombinant protein of mouse full-length mitochondrial elongation factor 1 (Mief1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Mitochondrial outer membrane protein which regulates mitochondrial fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity and DNM1L oligomerization. Binds ADP and can also bind GDP, although with lower affinity. Does not bind CDP, UDP, ATP, AMP or GTP. Inhibits DNM1L GTPase activity in the absence of bound ADP. Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP. Does not require ADP for its function in recruiting DNM1L.
• Molecular Mass: 51.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mief1 mitochondrial elongation factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: Mief1
• Synonyms: Mief1; mitochondrial elongation factor 1; Smc; Smcr7l; AI452372; A230016E22; mitochondrial dynamics protein MID51; Smith-Magenis syndrome chromosome region, candidate 7-like; mitochondrial dynamic protein MID51; mitochondrial dynamic protein of 51 kDa homolog; mitochondrial dynamics protein of 51 kDa homolog
• Gene ID: 239555
• mRNA Refseq: NM_178719
• Protein Refseq: NP_848834
• UniProt ID: Q8BGV8