Recombinant Mouse Mlh1 Protein, Myc/DDK-tagged

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Recombinant Mouse Mlh1 Protein, Myc/DDK-tagged

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Cat.No. : Mlh1-4089M
Product Overview : Purified recombinant protein of mouse full-length mutL homolog 1 (Mlh1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
Description : Heterodimerizes with Pms2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (Msh2-Msh6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of Pms2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (Mlh1-Pms2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with Mlh3 to form MutL gamma which plays a role in meiosis.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 84.7 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Mlh1 mutL homolog 1 [ Mus musculus (house mouse) ]
Official Symbol : Mlh1
Synonyms : MLH1; mutL homolog 1 (E. coli); DNA mismatch repair protein Mlh1; mutL protein homolog 1; colon cancer, nonpolyposis type 2; AI317206; AI325952; AI561766; 1110035C23Rik
Gene ID : 17350
mRNA Refseq : NM_026810
Protein Refseq : NP_081086
UniProt ID : Q9JK91
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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