Active Recombinant Mouse Mmp2 protein, His-tagged
Cat.No. : | Mmp2-1783M |
Product Overview : | Active Recombinant Mouse Mmp2 protein(P33434)(110~662 aa), fused with N-terminal His tag, was expressed in E.coli. |
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Species : | Mouse |
Source : | E.coli |
Tag : | His |
Protein Length : | 110-662 aa |
Form : | Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : | MMP2 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family .It is a gelatinase A, 72kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of MMP2 were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1 mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP2 protein as negative control, and blood sample as positive control. |
Molecular Mass : | 63.5kDa |
Endotoxin : | <1.0EU per 1μg (determined by the LAL method). |
Purity : | > 95% as determined by SDS-PAGE. |
Storage : | Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : | Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
Gene Name | Mmp2 matrix metallopeptidase 2 [ Mus musculus ] |
Official Symbol | Mmp2 |
Synonyms | MMP2; matrix metallopeptidase 2; 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2; GelA; Clg4a; MMP-2; |
Gene ID | 17390 |
mRNA Refseq | NM_008610 |
Protein Refseq | NP_032636 |
◆ Recombinant Proteins | ||
MMP2-29435TH | Recombinant Human MMP2, His-tagged | +Inquiry |
MMP2-095H | Recombinant Human matrix metallopeptidase 2 Protein, His&Flag tagged | +Inquiry |
MMP2-5051H | Recombinant Human MMP2, His-tagged | +Inquiry |
MMP2-4564H | Recombinant Human MMP2 protein, His-tagged | +Inquiry |
MMP2-220H | Active Recombinant Human MMP2 Protein (Tyr120-Cys660), C-His tagged, Animal-free, Carrier-free | +Inquiry |
◆ Native Proteins | ||
MMP2-46H | Native Human MMP-2 | +Inquiry |
MMP2-8455M | Native Mouse MMP2 | +Inquiry |
MMP2-47H | Native Human MMP-2/TIMP-2 Complex | +Inquiry |
MMP2-29475TH | Native Human MMP2 | +Inquiry |
◆ Cell & Tissue Lysates | ||
MMP2-2380HCL | Recombinant Human MMP2 cell lysate | +Inquiry |
Not For Human Consumption!
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