Recombinant Mouse Mov10 Protein, Myc/DDK-tagged

• Cat.No.: Mov10-4121M
• Product Overview: Purified recombinant protein of mouse full-length Mov10 RISC complex RNA helicase (Mov10), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Probable RNA helicase. Required for miRNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC. In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2. Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7. Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol. May function as a messenger ribonucleoprotein (mRNP) clearance factor.
• Molecular Mass: 114 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Mov10 Mov10 RISC complex RNA helicase [ Mus musculus (house mouse) ]
• Official Symbol: Mov10
• Synonyms: MOV10; Moloney leukemia virus 10; putative helicase MOV-10; moloney leukemia virus 10 protein; C77703; Mov-10
• Gene ID: 17454
• mRNA Refseq: NM_001163441
• Protein Refseq: NP_001156913
• UniProt ID: P23249