| Description : |
5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs. Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2. Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1. Facilitates LINE-1 uridylation by TUT4 and TUT7. Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol. May function as a messenger ribonucleoprotein (mRNP) clearance factor. Exhibits antiviral activity against dengue virus (DENV). |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
113.7 kDa |
| AA Sequence : |
MPSKFSCRQLREAGQCFESFLVVRG LDMETDRERLRTIYNRDFKISFGTP APGFSSMLYGMKIANLAYVTKTRVR FFRLDRWADVRFPEKRRMKLGSDIS KHHKSLLAKIFYDRAEYLHGKHGVD VEVQGPHEARDGQLLIRLDLNRKEV LTLRLRNGGTQSVTLTHLFPLCRTP QFAFYNEDQELPCPLGPGECYELHV HCKTSFVGYFPATVLWELLGPGESG SEGAGTFYIARFLAAVAHSPLAAQL KPMTPFKRTRITGNPVVTNRIEEGE RPDRAKGYDLELSMALGTYYPPPRL RQLLPMLLQGTSIFTAPKEIAEIKA QLETALKWRNYEVKLRLLLHLEELQ MEHDIRHYDLESVPMTWDPVDQNPR LLTLEVPGVTESRPSVLRGDHLFAL LSSETHQEDPITYKGFVHKVELDRV KLSFSMSLLSRFVDGLTFKVNFTFN RQPLRVQHRALELTGRWLLWPMLFP VAPRDVPLLPSDVKLKLYDRSLESN PEQLQAMRHIVTGTTRPAPYIIFGP PGTGKTVTLVEAIKQVVKHLPKAHI LACAPSNSGADLLCQRLRVHLPSSI YRLLAPSRDIRMVPEDIKPCCNWDA KKGEYVFPAKKKLQEYRVLITTLIT AGRLVSAQFPIDHFTHIFIDEAGHC MEPESLVAIAGLMEVKETGDPGGQL VLAGDPRQLGPVLRSPLTQKHGLGY SLLERLLTYNSLYKKGPDGYDPQFI TKLLRNYRSHPTILDIPNQLYYEGE LQACADVVDRERFCRWAGLPRQGFP IIFHGVMGKDEREGNSPSFFNPEEA ATVTSYLKLLLAPSSKKGKARLSPR SVGVISPYRKQVEKIRYCITKLDRE LRGLDDIKDLKVGSVEEFQGQERSV ILISTVRSSQSFVQLDLDFNLGFLK NPKRFNVAVTRAKALLIIVGNPLLL GHDPDWKVFLEFCKENGGYTGCPFP AKLDLQQGQNLLQGLSKLSPSTSGP HSHDYLPQEREGEGGLSLQVEPEWR NELTRTRPLEQKLISEEDLAANDIL DYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
