Active Recombinant Mouse Msln protein(Primary Amine Labeling), His-tagged, Biotinylated, Low Endotoxin

• Cat.No.: Msln-267M
• Product Overview: Biotinylated Recombinant Mouse Msln protein(Q61468-1)(298-600 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 298-600 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human CA125, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.79μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 90% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Msln mesothelin [ Mus musculus ]
• Official Symbol: Msln
• Synonyms: MSLN; mesothelin; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; MPF
• Gene ID: 56047
• mRNA Refseq: NM_018857
• Protein Refseq: NP_061345