Recombinant Mouse Muc16 Protein, His-tagged

• Cat.No.: Muc16-11021M
• Product Overview: Mouse CA125 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Asn 7750 - Pro 8422 (Accession # A0A140LJ72-1). This protein carries a polyhistidine tag at the C-terminus. Predicted N-terminus: Asn 7750

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 7750-8422 aa
• Description: The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Molecular Mass: The protein has a calculated MW of 76.7 kDa.
• Endotoxin: Less than 1.0 EU/μg by the LAL method/rFC method.
• Purity: >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Muc16 mucin 16 [ Mus musculus ]
• Official Symbol: Muc16
• Synonyms: MUC16; mucin 16; mucin-16; Ca125; Gm1480; 1110008I14Rik
• Gene ID: 73732
• mRNA Refseq: NM_001378248
• Protein Refseq: NP_001365177
• UniProt ID: A0A140LJ72