Recombinant Mouse Ndufa2 Protein, Myc/DDK-tagged

• Cat.No.: Ndufa2-4329M
• Product Overview: Purified recombinant protein of mouse full-length NADH:ubiquinone oxidoreductase subunit A2 (Ndufa2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a subunit of the NADH-ubiquinone oxidoreductase (complex I) enzyme, which is a large, multimeric protein. It is the first enzyme complex in the mitochondrial electron transport chain and catalyzes the transfer of electrons from NADH to the electron acceptor ubiquinone. The proton gradient created by electron transfer drives the conversion of ADP to ATP. The human ortholog of this gene has been characterized, and its structure and redox potential is reported to be similar to that of thioredoxins. It may be involved in regulating complex I activity or assembly via assistance in redox processes. In humans, mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. A pseudogene of this gene is located on chromosome 5.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 10.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ndufa2 NADH:ubiquinone oxidoreductase subunit A2 [ Mus musculus (house mouse) ]
• Official Symbol: Ndufa2
• Synonyms: NDUFA2; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; CI-B8; complex I-B8; NADH-ubiquinone oxidoreductase B8 subunit; B8; C1-B8; AV000592
• Gene ID: 17991
• mRNA Refseq: NM_010885
• Protein Refseq: NP_035015
• UniProt ID: Q9CQ75