Recombinant Mouse NEFL Protein (2-543 aa), His-tagged

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Recombinant Mouse NEFL Protein (2-543 aa), His-tagged

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Cat.No. : NEFL-1472M
Product Overview : Recombinant Mouse NEFL Protein (2-543 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.
Description : Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Source : Yeast
Species : Mouse
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 63.4 kDa
Protein length : 2-543 aa
AA Sequence : SSFGYDPYFSTSYKR RYVETPRVHISSVRS GYSTARSAYSSYSAP VSSSLSVRRSYSSSS GSLMPSLENLDLSQV AAISNDLKSIRTQEK AQLQDLNDRFASFIE RVHELEQQNKVLEAE LLVLRQKHSEPSRFR ALYEQEIRDLRLAAE DATNEKQALQGEREG LEETLRNLQARYEEE VLSREDAEGRLMEAR KGADEAALARAELEK RIDSLMDEIAFLKKV HEEEIAELQAQIQYA QISVEMDVSSKPDLS AALKDIRAQYEKLAA KNMQNAEEWFKSRFT VLTESAAKNTDAVRA AKDEVSESRRLLKAK TLEIEACRGMNEALE KQLQELEDKQNADIS AMQDTINKLENELRS TKSEMARYLKEYQDL LNVKMALDIEIAAYR KLLEGEETRLSFTSV GSITSGYSQSSQVFG RSAYSGLQSSSYLMS ARSFPAYYTSHVQEE QTEVEETIEATKAEE AKDEPPSEGEAEEEE KEKEEGEEEEGAEEE EAAKDESEDTKEEEE GGEGEEEDTKESEEE EKKEESAGEEQVAKK KD
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : Nefl neurofilament, light polypeptide [ Mus musculus ]
Official Symbol : NEFL
Synonyms : NEFL; Nfl; NF-L; NF68; CMT2E; AI847934;
Gene ID : 18039
mRNA Refseq : NM_010910
Protein Refseq : NP_035040
UniProt ID : P08551

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