Recombinant Mouse Neil2 Protein, Myc/DDK-tagged

• Cat.No.: Neil2-4366M
• Product Overview: Purified recombinant protein of mouse full-length nei like 2 (E. coli) (Neil2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 36.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Neil2 nei like 2 (E. coli) [ Mus musculus (house mouse) ]
• Official Symbol: Neil2
• Synonyms: NEIL2; nei like 2 (E. coli); endonuclease 8-like 2; nei homolog 2; nei-like protein 2; endonuclease VIII-like 2; DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; NEH2; Gm1212
• Gene ID: 382913
• mRNA Refseq: NM_201610
• Protein Refseq: NP_963904
• UniProt ID: Q6R2P8