Recombinant Mouse Nelfa Protein, Myc/DDK-tagged

• Cat.No.: Nelfa-4371M
• Product Overview: Purified recombinant protein of mouse full-length negative elongation factor complex member A, Whsc2 (Nelfa), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 57.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nelfa negative elongation factor complex member A, Whsc2 [ Mus musculus (house mouse) ]
• Official Symbol: Nelfa
• Synonyms: Nelfa; negative elongation factor complex member A, Whsc2; Nel; Whsc; Whsc2; Whsc2h; negative elongation factor A; Wolf-Hirschhorn syndrome candidate 2 homolog
• Gene ID: 24116
• mRNA Refseq: NM_011914
• Protein Refseq: NP_036044
• UniProt ID: Q8BG30